6942351e-4987-4a71-8ee4-d32d7a71122d_MovieS1.mov (78.93 MB)
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Supplementary Movie S1. Serial sectioning in a microtome with ultraLM fluorescence imaging.

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posted on 13.12.2016, 12:09 by Elisabeth Brama, Christopher J. Peddie, Gary Wilkes, Yan Gu, Lucy M. Collinson, Martin L. Jones

Slices acquired at 500 nm thickness with the ultraLM mounted on an ultramicrotome. Cells below the surface initially appear as blurred objects, come into focus as they approach the cutting plane and then disappear as they are physically removed from the block by the diamond knife.