Supplementary Movie S2. Serial blockface fluorescence imaging in SBF-SEM with miniLM.
Elisabeth Brama
Christopher J. Peddie
Gary Wilkes
Yan Gu
Lucy M. Collinson
Martin L. Jones
10.6084/m9.figshare.4291586.v1
https://wellcomeopenresearch.figshare.com/articles/media/Supplementary_Movie_S2_Serial_blockface_fluorescence_imaging_in_SBF-SEM_with_miniLM_/4291586
<p>Slices acquired at 100 nm thickness with the miniLM
operating inside the scanning electron microscope. The outer circle shows the
edge of the fibre bundle and the effect of the discrete fibre cores is seen as
a fixed-pattern pixellation across the surface.</p>
2016-12-13 12:09:17
GFP
In-resin fluorescence
Correlative
CLEM
Integrated
Ultramicrotome
Serial block face SEM
ultraLM
miniLM
Smart tracking
Locator tool
Cell Biology